Hematocrit Determination

This application note provides information on specific application methods and the use of Hettich products.

Excerpt:

Determination of the hematocrit value by centrifugation

The hematocrit value indicates the volumetric proportion of red blood cells in the blood. Centrifugation is the reference method for the determination of this value. It is used to separate the cellular components of the blood from the fluid ones and to package them tightly together. The reference method is described in DIN 58933-11). According to it, glass capillaries are centrifuged until the product of the minimum relative centrifugal force (RCF > 5000) acting on the erythrocytes and the numerical value for the centrifuging time in minutes is at least 100 000.

Hettich products are designed to help you achieve optimal results for your application and are built to perform to the specifications outlined in the operators manual. For application-specific information and settings, please refer to your organization’s standard operating procedure. As always, our Hettich representatives are here to help determine which Hettich products and/or accessories best fit your laboratory requirements.