In Molecular Biology, PCR (polymerase chain reaction) is used to amplify pieces of DNA through a number of cycles into millions of copies of the DNA sequence. The technique relies on Thermal Cycling, which consists of a heating and cooling sequence to separate the DNA double helix into single strands for amplification...
Read MoreHettich centrifuges in environmental analysis Determination of chlorophyll content as an indicator of water purity You can centrifuge water samples to determine chlorophyll and phaeophytin content. The concentration of chlorophyll permits a statement on water quality. PROCEDURE* The plant mass present in a water sample is first enriched through filtration. It is...
Read MoreDetermination of Sulfate Concentration for Chrome Baths Hettich centrifuges can be used to determine the sulfate concentration in chrome baths. To ensure a high-quality result when using chrome baths, their sulfuric acid concentration must be maintained at an optimum level. This concentration will change over time with the use of a chrome...
Read MoreSlide preparations for urine cytology Preparation of native urine To derive a good cytological preparation from a urine sample, it is not sufficient to centrifuge native urine onto commercial microscope slides. Urine has a low cell concentration, and those cells present adhere poorly to the slides. This would result in preparation with...
Read MoreDetermination of the Water Retention Value (WRV) in Pulp and Paper Manufacturing The water retention value (WRV) is an essential parameter in the manufacture of pulp and paper. It is an indication of the ability of a sample of wood fiber or pulp mass to retain water. The WRV changes in the...
Read MoreSlide preparations for the cytology of pleural fluid or ascitic fluid In addition to mesothelial cells, pleural fluid and ascitic fluid also contain lymphocytes and eosinophilic and neutrophilic granulocytes. The protein content is frequently around 30 g/l. The cell content often varies considerably. Both fluids may contain blood, and ascitic fluid may...
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